Journal: Genome biology
Article Title: Highly recurrent CBS epimutations in gastric cancer CpG island methylator phenotypes and inflammation.
doi: 10.1186/s13059-021-02375-2
Figure Lengend Snippet: Fig. 2 CBS epimutations associate with CIMP in primary GCs. a Overlap of genes that are promoter hypermethylated and downregulated at the RNA level in the discovery GC cell line panel, TCGA-stomach adenocarcinoma [STAD] and Singapore [SG] cohorts (β-value difference ≥0.3 and q-value < 0.05) [left panel]. Average promoter methylation β-values and gene expression of CBS gene in STAD according to CIMP subtypes (*P < 0.001, two-tailed Wilcoxon rank sum test, each CIMP group vs. non-CIMP group) [right panel]. b Immunohistochemistry of CBS in a normal human stomach with black arrow indicating cytoplasmic staining in epithelial cells. Control sections were not treated with the primary antibody. c Summary of CBS staining in 66 cases of matched normal and gastric adenocarcinomas (*P < 0.05, two- tailed Fisher’s exact test) [left panel] and an example of a matched normal vs. tumor case with a negative score [right panel]. d Summary of somatic and germline genetic alterations at CBS in STAD. PALP, pyridoxal- phosphate dependent enzyme domain; CBS, cystathionine beta-synthase domain; aa, amino acid
Article Snippet: Normal human stomach antrum and fundus tissue slides were purchased from Novus Biologicals (NBP2-30203, NBP230204).
Techniques: Methylation, Gene Expression, Two Tailed Test, Immunohistochemistry, Staining, Control